Microorganisms such as bacteria and fungi play essential roles in many application fields, like biotechnique, medical technique and industrial domain. Microorganism counting techniques are crucial in microorganism analysis, helping biologists and related researchers quantitatively analyze the microorganisms and calculate their characteristics, such as biomass concentration and biological activity. However, traditional microorganism manual counting methods, such as plate counting method, hemocytometry and turbidimetry, are time-consuming, subjective and need complex operations, which are difficult to be applied in large-scale applications. In order to improve this situation, image analysis is applied for microorganism counting since the 1980s, which consists of digital image processing, image segmentation, image classification and suchlike. Image analysis-based microorganism counting methods are efficient comparing with traditional plate counting methods. In this article, we have studied the development of microorganism counting methods using digital image analysis. Firstly, the microorganisms are grouped as bacteria and other microorganisms. Then, the related articles are summarized based on image segmentation methods. Each part of the article is reviewed by methodologies. Moreover, commonly used image processing methods for microorganism counting are summarized and analyzed to find common technological points. More than 144 papers are outlined in this article. In conclusion, this paper provides new ideas for the future development trend of microorganism counting, and provides systematic suggestions for implementing integrated microorganism counting systems in the future. Researchers in other fields can refer to the techniques analyzed in this paper.
Structural and Multidisciplinary Optimization - The overall layout optimization design of an orbital propellant depot involves the optimization of shape, size, and positions of propellant tanks in... 相似文献
Computing environment is moving towards human-centered designs instead of computer centered designs and human's tend to communicate wealth of information through affective states or expressions. Traditional Human Computer Interaction (HCI) based systems ignores bulk of information communicated through those affective states and just caters for user's intentional input. Generally, for evaluating and benchmarking different facial expression analysis algorithms, standardized databases are needed to enable a meaningful comparison. In the absence of comparative tests on such standardized databases it is difficult to find relative strengths and weaknesses of different facial expression recognition algorithms. In this article we present a novel video database for Children's Spontaneous facial Expressions (LIRIS-CSE). Proposed video database contains six basic spontaneous facial expressions shown by 12 ethnically diverse children between the ages of 6 and 12 years with mean age of 7.3 years. To the best of our knowledge, this database is first of its kind as it records and shows spontaneous facial expressions of children. Previously there were few database of children expressions and all of them show posed or exaggerated expressions which are different from spontaneous or natural expressions. Thus, this database will be a milestone for human behavior researchers. This database will be a excellent resource for vision community for benchmarking and comparing results. In this article, we have also proposed framework for automatic expression recognition based on Convolutional Neural Network (CNN) architecture with transfer learning approach. Proposed architecture achieved average classification accuracy of 75% on our proposed database i.e. LIRIS-CSE. 相似文献
Complex products such as satellites, missiles, and aircraft typically have demanding requirements for dynamic data management and process traceability. The assembly process for these complex products involves high complexity, strong dynamics, many uncertainties, and frequent rework and repair, especially in the model development stage. Achieving assembly data management and process traceability for complex products has always been a challenge. A recently proposed solution involves one-to-one mapping of the corresponding physical entity, also known as the digital twin method. This paper proposes a digital twin-based assembly data management and process traceability approach for complex products. First, the dynamic evolutionary process of complex product assembly data was analyzed from three dimensions: granularity, period and version. Then, a framework of digital twin-based assembly data management and process traceability for complex products was constructed. Some core techniques are: 1) workflow-based product assembly data organization and version management; 2) synchronous modeling of the product assembly process based on digital twin; and 3) hierarchical management and traceability of product assembly data based on digital twin. On this basis, an algorithm flowchart for generating a product assembly data package was created, which includes product assembly data management, assembly process traceability, and generation of a product assembly data package. Furthermore, the Digital Twin-based Assembly Process Management and Control System (DT-APMCS) was designed to verify the efficiency of the proposed approach. Some aerospace-related assembly enterprises are currently using DT-APMCS and achieving satisfactory results. Finally, a summary of our work is given, and the future research work is also discussed. 相似文献
This paper presents a human–robot co-working system to be applied to industrial tasks such as the production line of a paint factory. The aim is to optimize the picking task with respect to manual operation in a paint factory. The use of an agile autonomous robot co-worker reduces the time in the picking process of materials, and the reduction of the exposure time to raw materials of the worker improves the human safety. Moreover, the process supervision is also improved thanks to a better traceability of the whole process. The whole system consists of a manufacturing process management system, an autonomous navigation system, and a people detection and tracking system. The localization module does not require the installation of reflectors or visual markers for robot operation, significantly simplifying the system deployment in a factory. The robot is able to respond to changing environmental conditions such as people, moving forklifts or unmapped static obstacles like pallets or boxes. The system is not tied to specific manufacturing orders. It is fully integrated with the manufacturing process management system and it can process all possible orders as long as their components are placed into the warehouse. Real experiments to validate the system have been performed in a paint factory by a real holonomic platform and a worker. The results are promising from the evaluation of performance indicators such as exposure time of the worker to raw materials, automation of the process, robust and safe navigation, and the assessment of the end-user. 相似文献
The wettability of 3 mol% Y2O3-stabilized ZrO2 (3YSZ) by molten Cu can be greatly improved by applying pulsed currents at 1373 K. The improvement was closely related to current polarity and influenced by duty cycle and frequency. When the Cu/3YSZ interface was under cathodic condition, the wettability was mainly improved by the formation of substoichiometric ZrO2-δ and metallic Zr at the interface. Increasing duty cycle caused the interface to change from forming protrusions to creating depression. Decreasing frequency further deepened the depression. In the opposite polarity, the adsorption and enrichment of oxygen reduced the solid-liquid and liquid-vacuum interfacial energies, thus improving the wettability. Only bubbles formed at the interface. The larger the duty cycle, the more rapidly bubbles formed and escaped. The effect of frequency at this polarity was weak. Overall, this work provides a novel and effective strategy for tailoring the wettability and interfacial chemistry between zirconia and metals. 相似文献
Understanding the spatiotemporal evolution of overburden deformation during coal mining is still a challenge in engineering practice due to the limitation of monitoring techniques. Taking the Yangliu Coal Mine as an example, a similarity model test was designed and conducted to investigate the deformation and failure mechanism of overlying rocks in this study. Distributed fiber optic sensing (DFOS), high-density electrical resistivity tomography (HD-ERT) and close-range photogrammetry (CRP) technologies were used in the test for comprehensive analyses. The combined use of the three methods facilitates the investigation of the spatiotemporal evolution characteristics of overburden deformation, showing that the mining-induced deformation of overburden strata was a dynamic evolution process. This process was accompanied by the formation, propagation, closure and redevelopment of separation cracks. Moreover, the key rock stratum with high strength and high-quality lithology played a crucial role in the whole process of overburden deformation. There were generally three failure modes of overburden rock layers, including bending and tension, overall shearing, and shearing and sliding. Shear failure often leads to overburden falling off in blocks, which poses a serious threat to mining safety. Therefore, real-time and accurate monitoring of overburden deformation is of great significance for the safe mining of underground coal seams. 相似文献